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KMID : 0364219950380040257
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Korean Journal of Zoology
1995 Volume.38 No. 4 p.257 ~ p.261
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Bacterial Community Analysis of Lake Soyang in Winter by Using 16s and 23s rRNA-targeted Probes
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Hong Sun-Hee
Byeon Myeong-Seop
Ahn Tae-Seok
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Abstract
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To scrntinize the bacterial community composition of Lake Soyang in winter, bacterial numbers belonging to Eubacteria, Proteobacteria and Cytophaga-Flavobacterium group were estimated by using 16S and 23S rRNA targeted oligonucleotide probes. Total bacterial numbers ranged from 0.7×10^(6) to 1.1×10^(6) cells ·ml^(-1), and vertical
profile of total bacteria showed a peak at 5m depth. The ratio of eubacteria to total bacteria were 34-90% and at 5m and 10m depths those were low exhibiting, 39 and 34%, respectively. The percentage of proteobacteria α-group ranged 10.8∼28.7%, β-group 4.5∼53.5%, γ-group 4.9∼35.5% and Cytophaga-Flavobacterium group 6.1∼21.1%. The dominant group were β-group at 0, 2 and 5m γ-group at 10 m , α-group at 30m and Cytophaga Flavobacterium group at 50m depth. In winter season, Lake Soyang can ge divided into three layer, 0∼2m, 5∼10m and 30∼50m, by the bacteria
community composition. By this method, new informations about aquatic ecosystem were developed.
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KEYWORD
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Bacterial community, biodiversity, Lake Soyang, rRNA targeted probe
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